The functional analysis of Hepatitis B virus X protein
Human hepatitis B virus (HBV) is a major pathogen for liver diseases including cirrhosis and hepatocellular carcinoma. Among the HBV proteins, X protein plays critical roles in HBV replication and liver oncogenesis. However, the promiscuous functions of HBx are not elucidated yet. We are trying to validate the various regulatory functions of HBx protein in transcriptional transactivation, signal transduction, and carcinogenesis.
The regulatory mechanisms of HSV-1 viral proteins
Herpes simplex virus type 1 (HSV-1) is a neurotropic virus which causes a variety of diseases in human. Among immediate early proteins of HSV-1, ICP27 is essential for viral replication and has multiple functions at transcriptional and post-transcriptional levels. We are dissecting the functional mechanism of ICP27 in the regulation of signal transduction, apoptosis, and viral reactivation. In addition, we also carry the functional analysis of ICP22 and glycoproteins of HSV-1.
Ribozyme and RNA interference
Ribozymes are catalytic RNA molecules which bind target RNA sequences and cleave them in a sequence specific manner. We are developing the highly efficient ribozymes which are suppressing the specific viral gene expressions.