Functional analysis of developmental genes in Aspergillus species
With the aids of various screening technologies such as proteome, DEG (differentially expressed gene) and real-time PCR analysis, we have identified developmental stage-specific genes possibly modulated by the regulatory factors, VeA, NsdD, and etc. in the model filamentous fungus Aspergillus nidulans. At present we are attempting several approaches such as forced mass mating and phospho-proteome analyses to identify novel factors for sexual development in opportunistic human pathogen Aspergillus fumigatus. We are performing the gene disruption with CRISPR/Cas9, suppression test, protein-protein interaction, and morphogenetic analyses in order to characterize function of the genes and regulation mode of gene-expressions in Aspergillus spp.