Heon Man Lim
  • Ph. D.
  • Heon Man Lim
  • Molecular Biology
  • N11-515
  • Laboratory of Molecular Biology (N11-510)
  • +82-42-821-6276
  • hmlim@cnu.ac.kr

Academic Career

  • Ph. D., 1989, University of Delaware, USA In the filed of Molecular Biology

Career

  • Post-Doc., 1989-1992, Biology, Caltech, USA
  • Visiting scientist, 1999-2001, NIH, USA

Research Interests

  • Two-level inhibition of galK expression by Spot 42: Degradation of mRNA mK2 and enhanced transcription termination before the galK

    Spot 42-mediated degradation of the galK-specific mRNA, mK2, and Spot 42-mediated Rho-dependent transcription termination at the end of galT. Because the concentration of Spot 42 drops during the transition period of the polarity type switch, these results demonstrate that type 1 polarity is the result of alleviation of Spot 42-mediated galK down-regulation.

  • Spot 42 binding to different gal mRNAs results in differential consequences in the galactose operon.

    Spot 42, small RNA of 109 nucleotide-long functions through base-pairing of the 3 Regions in its 5’ half to target mRNA sequences. The Region I, II and III form 8-10 uninterrupted base-pairings with corresponding sequences of the target gal mRNAs. Out of 6 gal mRNAs, 4, mK1, mM1, mK2, and mT1, harbor the same Spot 42 binding site in different locations relative to their ends. We investigated the role of base-pairings of the 3 Regions on gal mRNA production.

Selected Publication

  • Wang, X., Ji, S. C., Jeon, H. J., Lee, Y., & Lim, H. M. (2015) Two-level inhibition of galK expression by Spot 42: Degradation of mRNA mK2 and enhanced transcription termination before the galK gene.Proc Natl Acad Sci U S A 112(24):7581-7586.
  • Wang, X., Ji, S. C., Yun, S. H., Jeon, H. J., Kim, S. W., & Lim, H. M. (2014) Expression of Each Cistron in the gal Operon Can Be Regulated by Transcription Termination and Generation of a galK-Specific mRNA, mK2. J. Bacteriol. 196(14): 2598
  • Ji, S. C., Wang, X., Yun, S. H., Jeon, H. J., Lee H. J., & Lim, H. M. (2011) In vivo transcription dynamics of the galactose operon: a study on the promoter transition from P1 to P2 at onset of stationary phase. PLoS One, 6(3):e17646