Functional genomics study in chinese cabbage
Using Brassica rapa oligomer chips (Br300K and Br135K microarray) and RNA Seq, we have analyzed gene expression profiles and identified genes of interest from B. rapa and B. oleracea. Most genes of interest are genes involved in pollen fertility, freezing tolerance, drought stress, leaf color and high temperature tolerance.
Molecular marker development in Brassica
To assist molecular breeding of Brassica crops, we have developed gene-specific molecular markers that are related to flowering time, temperature-stress tolerance, and anthocyanin biosynthesis. Target genes for developing molecular markers have been selected by transcriptome analyses, and followed by genomic sequence analysis. We are currently identified many gene-specific PCR-markers and thousands of SNPs and SSRs.
Functional study on unknown genes
We have identified many genes that would regulate desirable agronomic traits as well as biological phenomena. To elucidate their function, we used transgenic technologies, biochemistry and genetic approaches. We are currently using Arabidopsis, B. napus and B. rapa transgenic plants.