Spot 42 binding to different gal mRNAs results in differential consequences in the galactose operon.
Spot 42, small RNA of 109 nucleotide-long functions through base-pairing of the 3 Regions in its 5’ half to target mRNA sequences. The Region I, II and III form 8-10 uninterrupted base-pairings with corresponding sequences of the target gal mRNAs. Out of 6 gal mRNAs, 4, mK1, mM1, mK2, and mT1, harbor the same Spot 42 binding site in different locations relative to their ends. We investigated the role of base-pairings of the 3 Regions on gal mRNA production.